Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells
Background Information
Histone deacetylase 6 (HDAC6) is member of the class II HDAC family, shich share significant sequence homology to the yeast HDA1 protein. HDAC6 is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of huntingtin aggregates and retard HIV1 infection, but may also promote invasive motility in tumor cells.
Product Information
Format
Purified
Presentation
In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol
Applications
Application
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.
Key Applications
Enzyme Assay
Application Notes
HDAC deacetylase assays
Biological Information
Purity
Purity >90% by SDS-PAGE
Source
Human HDAC6
Species
Human
Specific Activity
>2 U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Physical Form
Frozen solution
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC6 into separate tubes before storing at -80°C.
Packaging Information
Material Size
50 µg
原厂资料：
Key Spec Table
Key Applications
Species
EA
Human
Description
Catalogue Number
03-226
Brand Family
Upstate
Trade Name
Upstate
Description
HDAC6 (His-tag) human recombinant
Overview
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells
Background Information
Histone deacetylase 6 (HDAC6) is member of the class II HDAC family, shich share significant sequence homology to the yeast HDA1 protein. HDAC6 is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of huntingtin aggregates and retard HIV1 infection, but may also promote invasive motility in tumor cells.
Product Information
Format
Purified
Presentation
In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol
Applications
Application
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.
Key Applications
Enzyme Assay
Application Notes
HDAC deacetylase assays
Biological Information
Purity
Purity >90% by SDS-PAGE
Source
Human HDAC6
Species
Human
Specific Activity
>2 U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Physical Form
Frozen solution
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC6 into separate tubes before storing at -80°C.
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