RIPAb+? LSM14A

• 产品编号：merck-m-03-184      品牌：millipore       原厂货号：03-184
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Description：RIPAb+™ LSM14A - RIP Validated Antibody and Primer Set

Overview：RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
The LSM14A protein is an Sm-like protein. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.

Background Information：The LSM14A protein is an Sm-like protein. These proteins have been identified in a variety of organisms based on sequence homology with the members of the Sm protein family. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.

Presentation：Anti-LSM14A (rabbit polyclonal), . One vial containing 50 µg affinity purified polyclonal in buffer containing 0.1 M Tris-glycine, 0.150 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C. 
Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
RIP Primers, U6snRNA.
One vial containing 75 μL of 5 μM of each primer specific for human U6 snRNA. Store at -20°C.
FOR: CTC GCT TCG GCA GCA CA
REV: AAC GCT TCA CGA ATT TGC GT 

Key Applications：

• Western Blotting
• RNA Binding Protein Immunoprecipitation (RIP)

原厂资料：

Description：RIPAb+™ LSM14A - RIP Validated Antibody and Primer Set

Overview：RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
The LSM14A protein is an Sm-like protein. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.

Background Information：The LSM14A protein is an Sm-like protein. These proteins have been identified in a variety of organisms based on sequence homology with the members of the Sm protein family. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.

Presentation：Anti-LSM14A (rabbit polyclonal), . One vial containing 50 µg affinity purified polyclonal in buffer containing 0.1 M Tris-glycine, 0.150 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C. 
Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
RIP Primers, U6snRNA.
One vial containing 75 μL of 5 μM of each primer specific for human U6 snRNA. Store at -20°C.
FOR: CTC GCT TCG GCA GCA CA
REV: AAC GCT TCA CGA ATT TGC GT 

Key Applications：

• Western Blotting
• RNA Binding Protein Immunoprecipitation (RIP)

注意事项：

Application Notes

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from 293 cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-LSM14A antibody (Cat. # CS204313). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: LSM14A) and 293 whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-LSM14A antibody (Cat. # CS204313, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231)
Arrow indicates LSM14A. 
Western Blot Analysis: 
Representative lot data.
NIH/3T3 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-LSM14A (1:1000) dilution. 
Proteins were visualized using a Donkey Anti-Rabbit conjugated to HRP and a chemiluminescence detection system. 
Arrow indicates LSM14A (~58 kDa). 

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.