RIPAb+? Musashi 2

• 产品编号：merck-m-03-115      品牌：millipore       原厂货号：03-115
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Description:RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal.

Overview:RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.

Background Information:In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. MSI2 or Musashi2 is a member of an RNA-binding protein family that appears to play a central role in post transcriptional gene regulation. The MSI2 gene encodes a protein containing two conserved tandem RNA recognition motifs. MSI2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages.

Presentation

Anti-Musashi 2 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C.

Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. 
Store at -20°C.

RIP Primers, PTP4A1. One vial containing 75 μL of 5 μM of each primer specific for human PTP4A1. Store at -20°C.
FOR: ATC CAA CCA ATG CGA CCT TA
REV: AAG GCC AAT CAA GAA CAT GG

Key Applications

• Immunohistochemistry (Paraffin)
• RNA Binding Protein Immunoprecipitation (RIP)
• Western Blotting
• Flow Cytometry

原厂资料：

Application Notes

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 2 antibody. 
Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 2) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 2 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit.
Arrow indicates Musashi 2 (Please see figures).

Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
A previous lot was used in immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi 2 (Please see figures).

Western Blot Analysis: 
Representative lot data.
A 1:1,000-2,000 dilution of a previous lot of this antibody was used to detect Musashi 2 in human brain (A), rat brain (B), SW480 (C), and T47D (D) cell lysates (Please see figures).

Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.

注意事项：

Application Notes

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 2 antibody. 
Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 2) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 2 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit.
Arrow indicates Musashi 2 (Please see figures).

Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
A previous lot was used in immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi 2 (Please see figures).

Western Blot Analysis: 
Representative lot data.
A 1:1,000-2,000 dilution of a previous lot of this antibody was used to detect Musashi 2 in human brain (A), rat brain (B), SW480 (C), and T47D (D) cell lysates (Please see figures).

Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.