RIPAb+ p54nrb/NonO, clone 78-1-C6

• 产品编号：merck-m-03-113      品牌：millipore       原厂货号：03-113
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Description:RIPAb+ p54nrb/NonO - RIP Validated Antibody and Primer Set.

Overview:RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.

Background Information:p54nrb/NonO is a DNA and RNA binding protein, that binds the conventional octamer sequence in double stranded DNA, as well as single-stranded DNA and RNA at a site independent of the duplex site. P54nrb/NonO is also involved in pre-mRNA splicing.

Presentation:Anti-p54nrb/NonO (Mouse Monoclonal IgG). One vial containing 50 µg of protein G purified mouse monoclonal IgG in 50 µL of buffer containing (0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Normal Mouse IgG. One vial containing 125 μg purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, FOS. One vial containing 75 μL of 5 μM of each primer specific for human FOS. Store at -20°C.
FOR: GAG AGC TGG TAG TTA GTA GCA TGT TGA
REV: AAT TCC AAT AAT GAA CCC AAT AGA TTA GTT A

Key Applications:

• Immunohistochemistry
• RNA Binding Protein Immunoprecipitation (RIP)
• Immunoprecipitation
• Western Blotting

原厂资料：

Description:RIPAb+ p54nrb/NonO - RIP Validated Antibody and Primer Set.

Overview:RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.

Background Information:p54nrb/NonO is a DNA and RNA binding protein, that binds the conventional octamer sequence in double stranded DNA, as well as single-stranded DNA and RNA at a site independent of the duplex site. P54nrb/NonO is also involved in pre-mRNA splicing.

Presentation:Anti-p54nrb/NonO (Mouse Monoclonal IgG). One vial containing 50 µg of protein G purified mouse monoclonal IgG in 50 µL of buffer containing (0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Normal Mouse IgG. One vial containing 125 μg purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, FOS. One vial containing 75 μL of 5 μM of each primer specific for human FOS. Store at -20°C.
FOR: GAG AGC TGG TAG TTA GTA GCA TGT TGA
REV: AAT TCC AAT AAT GAA CCC AAT AGA TTA GTT A

Key Applications:

• Immunohistochemistry
• RNA Binding Protein Immunoprecipitation (RIP)
• Immunoprecipitation
• Western Blotting

注意事项：

Application Notes

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using either 0.5 µg of a normal mouse IgG or 0.5 µg of Anti-p54nrb/NONO antibody. Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-p54nrb/NONO) and HeLa whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p54nrb/NONO antibody (0.5 µg/mL). 
Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).

Western Blot Analysis: 0.5-2.0 μg/mL of a previous lot detected p54nrb/NonO in RIPA lysates from MCF-7 cells (Please see figures).

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect p54nrb/NonO in human breast tumor sections.

Immunoprecipitation: Reported by an independent lab to immunoprecipitate p54nrb/NonO.

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.