PGLS is an enzyme in the second step pentose phosphate pathway. 6-Phosphogluconolactonase is crucial for the synthesis of nucleotide sugars and NADPH, the key cause for decreasing power. PGLS transforms 6-phosphogluconolactone to 6-phosphogluconate.
PGLS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-258a.a.) and having a molecular mass of 29.7kDa.
PGLS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.