Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
IL-12 p40 human Recombinant produced in Nicotiana benthamiana plant is a glycosilated polypeptide chain containing 312 amino acids, 23-328 aa (molecular formula C1575H2420N424O490S12).
IL-12 p40 is fused to a 6-His-tag at the N-terminal having the total molecular mass of 35.5kDa however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDS PAGE under reducing conditions.
IL-12 p40 IS purified by standard chromatographic techniques.
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