The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or from 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.
Features - Benefits
Be Efficient: Allows use of hard-to-obtain precious samples. Have Confidence: Provides maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets. Save Effort: No need to further concentrate samples for use. Save Time: Rapid protocol and provided DNase reagents streamline laboratory processes.
Applications
Purified RNA is suitable for many common downstream assays.
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