Cat.# Z5200 contains sufficient reagents for 3 separate mRNA isolations, each from 1–5mg of total RNA. Cat.# Z5210 contains the same reagents as Cat.# Z5200, excluding the Magnetic Separation Stand. Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations, each from 100–1,000μg of total RNA. Cat.# Z5310 contains the same reagents as Cat.# Z5300, excluding the Magnetic Separation Stand.
The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.
Features - Benefits
Improved Productivity: Entire mRNA purification process can be completed in approximately 45 minutes.
Highly Pure mRNA: Due to the strength and selectivity of the interaction between streptavidin and biotin, mRNA bound to the biotinylated oligo(dT) is captured by streptavidin-coated magnetic particles.
Confidence in Your Applications: Isolated mRNA is suitable for use with in vitro translation, RT-PCR and cDNA synthesis.
Flexibility: Configurations for use with large or small amounts of cells and tissues.
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