DNase Stop Solution (DSA)【子货号:Z312C,包装:1 x 13.25ml】
Yellow Core Buffer【子货号:Z317A,包装:1 x 2.5ml】
MnCl2, 0.09M【子货号:Z318B,包装:1 x 750μl】
DNase I (lyophilized)【子货号:Z358A,包装:1 x 1 vial】
Beta-Mercaptoethanol【子货号:Z523A,包装:1 x 2ml】
描述:
说明
SV 96 Total RNA Isolation System (SV 96总RNA纯化系统)从组织、培养细胞中高通量地纯化完整的总RNA,方法简单、快速。一次纯化96个样品的总RNA可在1小时内完成,无需进行离心操作。该系统包含DNase处理步骤,以充分减少会干扰扩增反应的DNA污染。纯化中不使用苯酚:氯仿抽提及乙醇沉淀,最终得到的RNA产物中检测不到DNase残留。
The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.
Protocols are available for Beckman Coulter and PerkinElmer instruments.
Features - Benefits
Confidence in Results: The product is tested to ensure that purified RNA will perform optimally in RT-PCR. Unique Design: Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing. Flexibility: The system is designed for both manual and automated formats. Automation: Validated automated methods available at: www.promega.com/automethods/ Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/custom/