The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete using a liquid-handling workstation.
Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.
Features - Benefits
Improve Productivity: Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
Improve Real-Time PCR Performance: Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
Gain Confidence in Results: DNase I treatment is included to remove genomic DNA contamination.
Achieve Convenience: Robotic protocols require no user intervention once you start the automated robotic method.
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