ProtoBlot? II AP System with Stabilized Substrate, Human

产品编号：W3940 品牌：promega 原厂货号：W3940
产品分类：蛋白质检测与分析 > 蛋白质印迹分析 > 印迹检测试剂及试剂盒
应用分类：蛋白质研究 > 免疫印迹 > 免疫印迹检测 > AP检测

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其它组分：

Anti-Human IgG (H&L) AP Conjugate【子货号：S382B，包装：1 x 100μl】

Western Blue® Stabilized Substrate for Alkaline Phosphatase【子货号：S3841，包装：3 x 100ml】

Tween® 20【子货号：W383B，包装：1 x 5ml】

Blot-Qualified BSA【子货号：W383B，包装：1 x 10g】

描述：

说明

RProtoBlot^® II AP Systems with Stabilized Substrate(带有稳定底物的ProtoBlot^® II AP系统)用于快速、灵敏地检测固定于醋酸纤维膜或PVDF膜上的蛋白质或其它大分子抗原。蛋白质可以在电泳后直接从凝胶转移到膜上(Western blot)，也可以在溶液中直接结合(“点”杂交)。试剂盒中包含Western Express^® Fast Blotting Protocol (Western Express^® 快速杂交实验方案)，能够显著地缩短免疫检测所需要的时间。所有ProtoBlot^® II AP 系统都含有BSA作为稳定剂，0.05%叠氮化钠作为防腐剂。

特点

• 快速：Western Express ^®快速杂交实验方案易于使用，点杂交检测仅需30-45 分钟，Western blot 仅需1-2 小时。

• 方便：试剂盒包含Western Blue^®Stabilized Substrate for AP ( 用于AP 的Western Blue^®稳定底物)，是一即用型的BCIP/NBT 溶液，不需要配制底物溶液。

对于很多实验，AP 标记的二抗优于HRP 标记的二抗，因为：
• 灵敏度更高(10 倍)。

• 不被叠氮化物所抑制。

• 长期储存时，底物不易褪色。

• 适用于PVDF 和醋酸纤维膜的实验方案均有提供。

原厂资料：

The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express® Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection. All ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative.

Features - Benefits

Fast: Easy-to-use Western Express® Protocol allows the detection of dot blots in 30–45 minutes and the detection of Western blots in 1–2 hours.
Convenient: The system contains Western Blue® Stabilized Substrate for AP, which is a ready-to-use solution of BCIP/NBT. No reagent preparation is required for the substrate.

For many applications, AP conjugates are superior to HRP conjugates because they:
offer greater sensitivity (tenfold) of detection.
are not inhibited by azide.
use a substrate that is not prone to fading during long-term storage.
have protocols provided for both PVDF and nitrocellulose membranes.

References

Towbin, H. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 4350–4.
Burnette, W.N. (1981) Anal. Biochem. 112, 195–203.
Hawkes, R. et al. (1982) Anal. Biochem. 119, 142–7.
Larson, G. (1992) Promega Notes 35, 18–20.
Blake, M.S. et al. (1984) Anal. Biochem. 136, 175–9.