ADP-Glo? Kinase Assay + FES Kinase Enzyme System

产品编号：V9311 品牌：promega 原厂货号：V9311
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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1 each

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其它组分：

FES Kinase Enzyme System【子货号：V1981，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组全长人源 FES 。 FES 是一个编码蛋白酪氨酸激酶的原癌基因，该激酶不同于 c-Src 、 c-Abl 及其他非受体酪氨酸激酶。 FES 最初被认为是一些可转化的逆转录病毒原癌蛋白的细胞同源物。 FES 在调节细胞骨架重组和由内向外的细胞信号传导中发挥作用，后者伴随着受体配体相互作用、细胞基质相互作用与细胞 - 细胞相互作用。使用转基因小鼠模型进行的遗传分析表明 FES 在炎症反应与天然免疫应答中发挥着调控作用。 FES 调控巨噬细胞对 LPS 的天然免疫应答，在某种程度上是通过调节 TLR4 受体复合物的内化和下调而实现的。 ADP-Glo™ Kinase Assay （ ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： FES ， 10μg ( 人源，重组全长蛋白 ) ； MW : ~ 125 kDa 。 • 底物： Poly (4:1 Glu, Tyr) Peptide 。 • 其它组分：反应缓冲液， DTT ， MnCl₂ 。 FES NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		FES Kinase Enzyme System Product Information
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于–70°C ；其他组分储存于–20°C 。
参考文献		1. Smithgall, T.E. et al. (1998) Crit Rev Oncog. 9(1) , 43–62. 2. Greer, P. (2002) Nat Rev Mol Cell Biol. 3(4) , 278–89.
专利/免责声明		For Cat.# V9311: Patents Pending. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending. U.S. Pat. No. 7,741,067 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human FES was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FES is a proto-oncogene that encodes a protein-tyrosine kinase distinct from c-Src, c-Abl and other nonreceptor tyrosine kinases. FES was originally identified as the cellular homolog of several transforming retroviral oncoproteins. FES plays a role in regulating cytoskeletal rearrangements and inside-out signalling that accompany receptor ligand, cell matrix and cell-cell interaction. Genetic analysis using a transgenic mouse model implicates FES in the regulation of inflammation and innate immunity. FES modulates the innate immune response of macrophages to LPS challenge, in part, by regulating the internalization and downregulation of the TLR4 receptor complex.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: FES, 10μg (Human, recombinant full-length). MW: ~125kDa.
Substrate: Poly (4:1 Glu, Tyr) Peptide.
Other: Reaction Buffer, DTT, MnCl2.

FES NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Smithgall, T.E. et al. (1998) Crit Rev Oncog. 9(1), 43–62.
2.Greer, P. (2002) Nat Rev Mol Cell Biol. 3(4), 278–89.