ADP-Glo? Kinase Assay +CDK1/CyclinA2 Kinase Enzyme System

产品编号：V9211 品牌：promega 原厂货号：V9211
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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其它组分：

CDK1/CyclinA2 Kinase Enzyme System【子货号：V2961，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒共表达 N 端带 GST 标签的重组全长人源 CDK1 和 CyclinA2 。 CDK1 （细胞分裂调控蛋白 1 ）是完成 START 所必需的， START 是细胞分裂起始所必需的细胞周期调控事件。 CDK1 是一个蛋白激酶复合体的催化亚基，该复合体被称为 M 期促进因子，能诱导细胞进入分裂期，在真核细胞内广泛存在。用金丝桃素进行光动力治疗后，停滞于 G2/M 期的细胞内 Bcl-2 的磷酸化，参与了 CDK1 介导的信号传递，并延迟细胞凋亡的开始。已有研究表明 CDK 抑制剂 NU2058 对雄性激素非依赖性前列腺癌具有治疗潜力。 ADP-Glo^™ Kinase Assay （ ADP-Glo^™激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo^™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo^™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： CDK1/CyclinA2 , 10μg ( 人源，重组全长蛋白 ) ； MW : ~59kDa (CDK1) ； 78kDa (CyclinA2) 。 • 底物： Histone H1 —— 天然的组蛋白 H1 是从小牛胸腺组织中纯化得到。其它组分：反应缓冲液， DTT 。 CDK1/CyclinA2 NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo^™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo^™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		CDK1/CyclinA2 Application Notes. Kinase-Lot-Specific Data Sheets. ADP-Glo™ Kinase Assay Technical Manual #TM313.
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。
参考文献		1. Vantieghem, A. et al. (2002) J. Biol. Chem. 277(40) , 37718–31. 2. Rigas, A.C. et al. (2007) Oncogene 26 , 7611–9.
专利/免责声明		For Cat.# V9211: Patents Pending. U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer also has been demonstrated.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: CDK1/CyclinA2, 10μg (Human, recombinant full-length). MW: ~59kDa (CDK1); 78kDa (CyclinA2).
Substrate: Histone H1- Native histone H1 was purified from calf thymus tissues.
Other: Reaction Buffer, DTT.

CDK1/CyclinA2 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Vantieghem, A. et al. (2002) J. Biol. Chem. 277(40), 37718–31.
2.Rigas, A.C. et al. (2007) Oncogene 26, 7611–9.