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ADP-Glo? Kinase Assay +AXL Kinase Enzyme System

ADP-Glo? Kinase Assay +AXL Kinase Enzyme System

产品编号：V9171 品牌：promega 原厂货号：V9171
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
应用分类：

包装：

1 each

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其它组分：

AXL Kinase Enzyme System【子货号：V3961，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

说明

在Sf9 昆虫细胞中，我们通过杆状病毒表达N 端带His 标签的重组人源AXL （第473 位氨基酸 - 末端）。AXL 是受体酪氨酸激酶家族的成员，具有致癌潜力，与人粒细胞白血病有关。 AXL 是细胞增殖和分化调控复合体信号网络的一个成员。在NIH/3T3 细胞中，AXL cDNA 的过表达会诱导这些细胞的肿瘤转化，并伴随着大小为140kDa 的AXL 酪氨酸磷酸化蛋白的出现。AXL cDNA 在杆状病毒系统中的表达会引起适当的重组蛋白的表达，该蛋白被认为是抗磷酸酪氨酸抗体，这确认了 AXL 蛋白是磷酸化的酪氨酸。

ADP-Glo^™Kinase Assay （ADP-Glo^™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的ADP 含量：ADP 转化为ATP 后，ATP 即可作为Ultra-Glo^™ 萤光素酶催化反应的底物，产生光信号。发光信号与ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。ADP-Glo^™激酶检测试剂盒可用于检测几乎所有可生成ADP 的酶类 ( 如激酶或ATP 酶 ) 的活性，ATP 浓度可高达1 mM 。

激酶酶系统的组成：

• 激酶：AXL ，10μg ( 人源，重组，第473 位氨基酸 - 末端 ) ；MW : ~ 55 kDa 。

• 底物：Axltide （KKSRGDYMTMQIG ）；从小鼠胰岛素受体底物1 （第979 位 – 第989 位氨基酸）中衍生得到。

• 其它组分： 反应缓冲液，DTT 。

AXL NCBI 数据库入口。

人类激酶酶系统及其应用。

特点

• 在实验室内即可筛选更多的化合物：ADP-Glo^™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。

• 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的DTT 和 MnCl₂。

• 可得到可靠结果：ADP-Glo^™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

操作手册

AXL Application Notes.
Kinase-Lot-Specific Data Sheets.
ADP-Glo™ Kinase Assay Technical Manual #TM313.

储存条件

收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。

参考文献

1 O`Bryan, J.P. et al. (1991) Mol. Cell Biol. 11(10) , 5016–31.

2 Janssen, J.W. et al. (1991) Oncogene 6(11) , 2113–20.

专利/免责声明

For Cat.# V9171:

Patents Pending.

U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending.

U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending.

The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant human AXL (amino acids 473–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia. AXL is a member of a complex signaling network that is involved in the control of cell proliferation and differentiation. Overexpression of AXL cDNA in NIH/3T3 cells induces neoplastic transformation of these cells with the concomitant appearance of a 140kDa AXL tyrosine-phosphorylated protein. Expression of AXL cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by anti-phosphotyrosine antibodies, confirming that the AXL protein is tyrosine phosphorylated.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: AXL, 10μg (Human, recombinant; amino acids 473–end). MW: ~55kDa.
Substrate: Axltide (KKSRGDYMTMQIG); derived from the mouse Insulin receptor substrate 1 (amino acid 979–989).
Other: Reaction Buffer, DTT.

AXL NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.O'Bryan, J.P. et al. (1991) Mol. Cell Biol. 11(10), 5016–31.
2.Janssen, J.W. et al. (1991) Oncogene 6(11), 2113–20.