PSECTAG2/HYGROA,B,C20 UG

• 产品编号：V91020      品牌：invitrogen       原厂货号：V91020
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：

描述：

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:

• Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)

• Cytomegalovirus (CMV) promoter for high-level constitutive expression

• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody

• C-terminal c-myc epitope for detection with an Anti-myc Antibody

• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability

• SV40 origin for episomal replication and simple vector rescue in

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• cell lines expressing the large T antigen (e.g., COS-1, COS-7)

The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.

The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.