Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion.
All three pTracer™ vectors have the following features:
• Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines • BGH polyA signal and transcription termination sequences to enhance mRNA stability
The pTracer™-SV40 vector offers: • SV40 promoter for high-level constitutive expression of your gene of interest • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen
The pTracer™-CMV2 vector offers: • CMV promoter for high-level constitutive expression of your gene of interest • EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion • Ampicillin resistance gene for selection in E. coli
The pTracer™-EF vectors offer: • EF-1α promoter for high-level constitutive expression of your gene of interest • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion • C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody • C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody • Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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