The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. MagneGST™ particles are supplied as a 25% slurry and have a binding capacity of 5–10mg of GST protein per 1ml of settled resin.
Features - Benefits
Simple: One-step purification of multiple samples with easy handling. Quick: After cell lysis, no requirement for high-speed centrifugation to clear lysate. Scalable: Scalable protocol using 1–50ml of cell culture. Efficient: Achieve high yields with little or no nonspecific background.
Applications
Protein purification of glutathione-S-transferase fusion proteins from cleared or crude E. coli cell lysate.
High-throughput purification of GST-tagged proteins using automated platforms.
For more information, see the Protocols & Applications Guide.
Notes
This product requires the use of a magnetic stand.
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