MagneHis? Protein Purification System

• 产品编号：V8550      品牌：promega       原厂货号：V8550
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 配体耦联磁珠 > 标签蛋白去除和分离磁珠
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The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP; validated methods are available at: www.promega.com/automethods/
 

Features - Benefits

Simple: No centrifugation or vacuum is required once the cells are lysed.
Flexible: MagneHis™ Ni-Particles are compatible with a variety of common buffers.
Efficient: Binding capacity is up to 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.
 

Applications

Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
High-throughput purification of His- or HQ-tagged proteins using automated platforms.
Purification of His-tagged proteins from insect or mammalian cell lysates.
Pull-down of His-tagged proteins for protein:protein interaction studies.
For more information, see the Protocols & Applications Guide.
 

Notes

This product requires the use of a magnetic stand.