ADP-Glo? Kinase Assay +RET Kinase Enzyme System

• 产品编号：V8061      品牌：promega       原厂货号：V8061
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

其它组分：

描述：

Recombinant human RET (amino acids 658–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RET gene codes for a transmembrane tyrosine kinase, which is a subunit of a multimeric complex that acts as a receptor for four structurally related molecules: GDNF, neurturin, artemin and persephin. Germline mutations of RET cause a dominantly inherited dysgenesis of the enteric nervous system known as Hirschsprung's disease. RET is constitutively activated by point mutations in hereditary medullary thyroid carcinomas (MTCs). Several single nucleotide polymorphisms of the RET gene have been described. Multiple endocrine neoplasia type 2A (MEN 2A) have been reported to be associated with two mutations of the proto-oncogene RET.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: RET, 10μg (Human, recombinant; amino acids 658–end). MW: ~74kDa.
Substrate: IGF1Rtide (KKKSPGEYVNIEFG); derived from human IRS-1 protein residues 891–902.
Other: Reaction Buffer, DTT.

RET NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Geneste, O. et al. (1999) Hum. Mol. Genet. 8(11), 1989–99.
2.Tessitore, A. et al. (1999) J. Clin. Endocrinol. Metab. 84(10), 3522–7.

原厂资料：