•物有所值:当使用推荐的稀释度1:5,000 时,该产品可制备200ml blotting 溶液, 每次使用10ml,可足够完成20 次Western blot 实验。
原厂资料:
Anti-ACTIVE® MAPK pAb is a polyclonal rabbit antibody. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the mitogen-activated protein (MAP) kinase enzymes.
Features - Benefits
Specificity: Preferentially detects the dually phosphorylated, active form of the mitogen-activated protein kinase (MAPK) enzymes (ERK1 and ERK2). Immunogen: Dually phosphorylated Thr/Glu/Tyr region (pTEpY) derived from the catalytic core of the active form of the mitogen-activated protein kinase (MAPK) enzymes, ERK1 and ERK2, which corresponds to Thr183 and Tyr185 of the mammalian ERK2 enzyme. Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4). Value: When used at the recommended 1:5,000 dilution, this product will generate 200ml of blotting solution, sufficient for 20 Western blots of 10ml each.
Applications
(Suggested working dilution)
Western blotting (1:5,000).
Immunocytochemistry (1:500).
Immunohistochemistry (1:500).