pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
• The OpIE2 promoter for constitutive expression • The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein • The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks • C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
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For Research Use Only. Not for use in diagnostic procedures.