ADP-Glo? Kinase Assay + MINK1 Kinase Enzyme System

产品编号：V8001 品牌：promega 原厂货号：V8001
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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其它组分：

MINK1 Kinase Enzyme System【子货号：V3911，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-2-℃】

描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组人源 MINK1 （第 1 位 - 第 320 位氨基酸）。 MINK1 属于生发中心（germinal center ）激酶家族的成员，该家族与 Ste20 家族同源，调控包括细胞形态、细胞骨架重排、细胞存活在内的非常广泛的细胞过程。 MINK1 的致死性激酶突变体的过表达会导致细胞扩散增加、肌动蛋白应力纤维形成加速、细胞外基质的粘性增加、以及细胞运动能力和侵染能力的降低。当 Ras 通过活性氧参与的机制被诱导后， MINK 被激活，并介导了 Raf/ERK 信号通路下游的 p38 MAPK 的激活。 ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： MINK1 ， 10μg ( 人源，重组，第 1 位 - 第 320 位氨基酸 ) ； MW : ~ 61 kDa 。 • 底物：天然的猪髓磷脂碱性蛋白（ MBP ）。其它组分：反应缓冲液， DTT 。 MINK1 NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		MINK1 Kinase Enzyme System Product Information
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于–70°C ；其他组分储存于–20°C 。
参考文献		1. Hu, Y. et al. (2004) J. Biol. Chem. 279(52) , 54387–97. 2. Nicke, B. et al. (2005) Mol. Cell 20(5) , 673–85.
专利/免责声明		For Cat.# V8001: Patents Pending. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending. U.S. Pat. No. 7,741,067 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant human MINK1 (1–320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes, including cell morphology, cytoskeletal rearrangement and survival. Overexpression of kinase-dead mutants of MINK1 leads to enhanced cell spreading, actin stress fiber formation, adhesion to extracellular matrix and decreased cell motility and invasion. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of p38 MAPK downstream of the Raf/ERK pathway.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: MINK1, 10μg (Human, recombinant; amino acids 1–320). MW: ~61kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.

MINK1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Hu, Y. et al. (2004) J. Biol. Chem. 279(52), 54387–97.
2.Nicke, B. et al. (2005) Mol. Cell 20(5), 673–85.