每次分析使用 1 μ g 预消化型人类蛋白提取物，注射入仪器中 ( (Waters NanoAquity HPLC System interfaced to a Thermo Fisher Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer ) 。以人类蛋白提取物每周监测仪器分析性能的稳定性。
原厂资料：
The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications (i.e., instrument monitoring; Figure 1). The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation. The yeast extracts are beneficial for users who prefer working with a relatively compact and well studied proteome, whereas the human extract provides opportunity for working with a complex proteome having a large dynamic range. Consistent extract protein composition is ensured by tight control over cell culture conditions and manufacturing process.
Lot-to-lot consistency of extracts is monitored by various protein and peptide qualitative and quantitation methods, including LC/MS and amino acid analysis (Table 1). Our manufacturing process assures compatibility with reverse phase liquid chromatography and mass spectrometry by monitoring nonspecific protein fragmentation, nonbiological post-translational modifications and, for digested extracts, minimal undigested peptides (Table 1).
Features - Benefits
•Compatible with LC/MS instrumentation platforms.
•Minimal nonbiological post-translational modifications.
•Peptide quantity measured by AAA.
•Model systems for method development/instrument monitoring.
•Multiple formats (digest/intact).
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