描述：
Recombinant human SIK (1–303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on Thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: SIK, 10μg (Human, recombinant; amino acids 1–303). MW: ~36kDa.
Substrate: AMARA peptide (AMARAASAAALARRR).
Other: Reaction Buffer, DTT.
SIK NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Features - Benefits
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
Takemori, H. et al. (2007) TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 274, 3202–9.
Sjostrom, M. (2007) SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. USA 104, 16922–7.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
