描述:
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues, being most abundant in hematopoietic tissues, spleen, thymus and peripheral blood leukocytes, as well as in testis, small intestine and colon (1). It is highly expressed in human leukemic, lymphoma and colorectal adenocarcinoma cell lines. This suggests a role for PIM2 in proliferating cells as well as during meiosis. Similar to PIM1, PIM2 also acts as a pro-survival kinase, and BAD protein is a legitimate PIM2 substrate (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: PIM2, 10μg (Human, recombinant full-length). MW: ~61kDa.
Substrate: S6K Substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acids 230–238).
Other: Reaction Buffer, DTT.
PIM2 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Features - Benefits
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Baytel, D. et al. (1998) The human Pim-2 proto-oncogene and its testicular expression. Biochim. Biophys. Acta 1442, 274–85.
2.Yan, B. et al. (2003) The PIM-2 kinase phosphorylates BAD on serine 112 and reverses BAD-induced cell death. J Biol Chem. 278, 45358–67.