DNA-Dependent Protein Kinase

• 产品编号：V5811      品牌：promega       原厂货号：V5811
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白激酶
• 应用分类：

描述：

 DNA- 依赖的蛋白激酶 (DNA-PK) 可以在体外使得多种DNA-结合底物磷酸化, 包括肿瘤抑制蛋白p53、SV40 大T 抗原和多种转录因子。DNA-PK 在基因调控和细胞生长中发挥重要作用。

        DNA-PK 从HeLa 细胞核提取物中分离，是由 400kDa 的催化亚基与被命名为 Ku 的155kDa DNA 结合亚基( 后者又由大约85kDa和70kDa 两部分组成) 组成的异型二聚体。

原厂资料：

DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors. DNA-PK is thought to play a role in controlling gene regulation and cell growth.
DNA-PK is isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa.