Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites (Figure 1).
Replacing trypsin with Trypsin/Lys-C Mix in this conventional protocol leads to multiple benefits for protein analysis including more accurate mass spectrometry-based protein quantitation and improved protein mass spectrometry analytical reproducibility. Trypsin/Lys-C Mix also provides greater tolerance to trypsin-inhibiting agents, assuring efficient digestion of proteins for which protein purification is limited or not feasible.
Features - Benefits
Simple to Use:Use standard overnight digestion with nondenaturing conditions.
Enhanced Proteolysis:Increase peptide recovery, resulting in enhanced protein quantitation and improved reproducibility and eliminating the majority of missed cleavages (Figures 1 and 2).
Tolerant to Trypsin-Inhibiting Contaminants:Generate mass spectrometry data from poor-quality sample material (Figure 3).
Applications
In-solution protein digestion.
In-solution digestion of proteolytically resistant proteins.
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