The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the periplasmic space. The vector provides:
• The araBAD promoter for tightly regulated expression • Translation initiation signals for optimized E. coliexpression • Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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