ADP-Glo? Kinase Assay +PDGFRα (T674I) Kinase Enzyme System

• 产品编号：V4487      品牌：promega       原厂货号：V4487
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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描述：

Recombinant human PDGFRα (T674I) (550–end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFRα (platelet-derived growth factor receptor α) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. Aberrant expression of PDGFRα has been linked to developmental abnormalities in vertebrate models and implicated in multiple disease states in humans. There is widespread expression of PDGFRα in renal cell types involved in fibrotic and sclerosing processes (1). PDGF and its receptor PDGFRα are inducers of fibrosis in the repair phase of inflammatory bowel disease, and they may also be involved in the active inflammatory phase (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: PDGFRα (D842V), 10μg (Human, recombinant; amino acids 550–end). MW: ~95kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, MnCl2, DTT.

PDGFRα (T674I) NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Floege, J. et al. (1998) Expression of PDGF alpha-receptor in renal arteriosclerosis and rejecting renal transplants. J Am Soc Nephrol. 9, 211–23.
2.Kumagai, S. et al. (2001) Platelet-derived growth factor and its receptors are expressed in areas of both active inflammation and active fibrosis in inflammatory bowel disease. Tohoku J Exp Med. 195, 21–33.

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