ADP-Glo? Kinase Assay + CK2α 1 Kinase Enzyme System

产品编号：V4483 品牌：promega 原厂货号：V4483
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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包装：

1 each

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其它组分：

CK2α1 Kinase Enzyme System【子货号：V4482，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

Recombinant full-length human CK2α1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2α1 is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth. It is highly expressed in a lympho-proliferative disease of cattle and in many human cancers. Overexpression of the CK2 catalytic subunit in lymphocytes of transgenic mice leads to T cell lymphoma (1). The highest CK2α1 activity is found in mouse testicles and brain, followed by spleen, liver, lung, kidney and heart (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: CK2α1, 10μg (Human, recombinant full-length). MW: ~70kDa.
Substrate: Native Casein Protein purified from bovine milk.
Other: Reaction Buffer, DTT.

CK2α1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Rifkin, I.R. et al. (1998) Acceleration of lymphoproliferative and autoimmune disease by transgenic protein kinase CK2 alpha. J. Immunol. 161, 5164–70.
2.Guerra, B. et al. (1999) Protein kinase CK2: evidence for a protein kinase CK2beta subunit fraction, devoid of the catalytic CK2alpha subunit, in mouse brain and testicles. FEBS Lett. 462, 353–7.