ADP-Glo? Kinase Assay + TNIK Kinase Enzyme System

• 产品编号：V4159      品牌：promega       原厂货号：V4159
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

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描述：

Recombinant human TNIK (1–367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serve a regulatory function (1). TNIK is mainly expression in brain, heart and spleen and is a specific effector of RAP2, which regulates actin cytoskeleton (2). TNIK is autophosphorylated in a manner dependent upon Lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: TNIK, 10μg (Human, recombinant; amino acids 1–367). MW: ~67kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT. MnCl2.

TNIK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

Fu, C.A. et al. (1999) TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton. J. Biol. Chem. 274, 30729–37.
Taira, K. et al. (2004) The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton. J. Biol. Chem. 279, 49488–96.

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