The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophilaactin 5C gene. The pAc5.1/V5-His vector also offers the following features:
• Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
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注意事项:
For Research Use Only. Not for use in diagnostic procedures.