ADP-Glo? Kinase Assay +DNA-PK Kinase Enzyme System

产品编号：V4107 品牌：promega 原厂货号：V4107
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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1 each

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其它组分：

DNA-PK Kinase Enzyme System【子货号：V4106，包装：1 x 2,500 units，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

Native human DNA-Dependent Protein Kinase (DNA-PK) purified from HeLa cells. DNA-PK consists of an approximately 460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and an 70kDa peptide (1). The gene sequence can be found at accession number NM_006904.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: DNA-PK, 2,500 units (Human, native full-length). MW: ~460kDa (catalytic subunit); ~85kDa (Ku subunit 1); ~70kDa (Ku subunit 2).
Substrate: DNA-Dependent Protein Kinase Peptide Substrate (EPPLSQEAFADLWKK).
Other: Reaction Buffer, DNA-PK Activation Buffer, DTT.

DNA-PK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Bulk quantities available upon request.

References

Gottlieb, T.M. and Jackson, S.P. (1993) The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen. Cell 72, 131–42.