ADP-Glo? Kinase Assay + TAOK1 Kinase Enzyme System

• 产品编号：V4091      品牌：promega       原厂货号：V4091
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

其它组分：

描述：

Recombinant human TAOK1 (1–314) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs) (1). The activation of and binding to MEK3 by TAOK1 implicates TAOK1 in the regulation of the p38-containing stress-responsive MAP kinase pathway. A microtubule affinity-regulating kinase kinase, TAOK1 (also known as MARKK) is an important regulator of mitotic progression, required for both chromosome congression and checkpoint-induced anaphase delay (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: TAOK1, 10μg (Human, recombinant; amino acids 1–314). MW: ~63kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.

TAOK1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Hutchison, M. et al. (1998) Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades. J. Biol. Chem. 273, 28625–32.
2.Draviam, V.M. et al. (2007) A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling. Nat. Cell Biol. 9, 556–64.

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