ADP-Glo? Kinase Assay + TAK1-TAB1 Kinase Enzyme System

• 产品编号：V4089      品牌：promega       原厂货号：V4089
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

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描述：

Recombinant human proteins TAK1 (1–303) and TAB1 (437–end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP) (1). In response to IL-1, TAK1 forms a kinase complex with TAB1, and this complex is required for the activation of nuclear factor kappa B (NF-κB). TAK1 also can activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. TAK1 is essential for thymocyte development and activation, and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate NF-κB and JNK and are prone to apoptosis upon stimulation.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: TAK1-TAB1, 10μg (Human, recombinant; TAK1 (1–303) and TAB1 (437–end)). MW: ~74kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.

TAK1 NCBI Database Entry. 
TAB1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Yamaguchi, K. et al. (1995) Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction. Science 270, 2008–11.
2.Liu, H.-H. et al. (2006) Essential role of TAK1 in thymocyte development and activation. Proc. Nat. Acad. Sci. USA 103, 11677–82.

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