ADP-Glo? Kinase Assay + STK33 Kinase Enzyme System

• 产品编号：V4087      品牌：promega       原厂货号：V4087
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

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描述：

Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. STK33 is a distant member of the CAMK group of serine/threonine kinases but it lacks the calcium/calmodulin-binding domain and the C-terminal regulatory tail. STK33 is highly expressed in testis, lung epithelia, alveolar macrophages, horizontal cells in the retina and in embryonic organs such as heart, brain and spinal cord. STK33 is essential for abnormal cell growth in human cell lines expressing oncogenic mutations in KRAS but not in human cancer cell lines expressing wildtype KRAS. STK33 is required for survival and proliferation of mutant KRAS-dependent cancer cells in which it suppresses the S6K1-BAD proapoptotic signaling pathway.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: STK33, 10μg (Human, recombinant full-length). MW: ~94kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.

STK33 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

Mujica A.O. et al. (2001) A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3. Gene 280, 175–81.
Scholl, C. et al. (2009) Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells. Cell 137, 821–34.

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