ADP-Glo? Kinase Assay + RIPK2 Kinase Enzyme System

• 产品编号：V4085      品牌：promega       原厂货号：V4085
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

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描述：

Recombinant human RIPK2 (1–299) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1, and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: RIPK2, 10μg (Human, recombinant; amino acids 1–299). MW: ~59kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.

RIPK2 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Inohara, N. et al. (1998) RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273, 12296–300. Erratum: (1998) J. Biol. Chem 273, 18675.
2.Thome, M. et al. (1998) Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8, 885–8.

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