ADP-Glo? Kinase Assay + PYK2 Kinase Enzyme System

产品编号：V4083 品牌：promega 原厂货号：V4083
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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包装：

1 each

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其它组分：

PYK2 Kinase Enzyme System【子货号：V4082，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-30℃】

描述：

Recombinant human PYK2 (360–690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family. PYK2/FAK2 can be activated by a variety of extracellular signals that elevate intracellular calcium concentration and by stress signals (1). Unlike FAK, which is widely expressed in various tissues and links transmembrane integrin receptors to intracellular pathways, PYK2/FAK2 is expressed mainly in the central nervous system and in cells derived from hematopoietic lineages. In osteoclasts, although FAK is expressed, PYK2/FAK2 appears to be the predominant mediator of integrin alpha(v)beta3 signaling events that influence osteoclast physiology and pathology (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: PYK2, 10μg (Human, recombinant; amino acids 360–690). MW: ~39kDa.
Substrate: Poly (4:1 Glu, Tyr) peptide.
Other: Reaction Buffer, DTT, MnCl2.

PYK2 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Avraham, H. et al. (2000) RAFTK/Pyk2-mediated cellular signalling. Cell Signal. 12, 123–33.
2.Xiong, W.C. et al. (2003) PYK2 and FAK in osteoclasts. Front Biosci. 8, d1219–26.