ADP-Glo? Kinase Assay + IKKα Kinase Enzyme System

• 产品编号：V4069      品牌：promega       原厂货号：V4069
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

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描述：

Full-length recombinant human IKKα was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKKα is a serine/threonine protein kinase that phosphorylates the I-κB protein, which is an inhibitor of the transcription factor NF-κB complex. Phosphorylation of I-κB protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-κB complex (1). IKKα is an essential regulator of NF-κB-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKα is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFNα production. IKKα is also involved in the epidermis, where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: IKKα, 10μg (Human, recombinant full-length). MW: ~114kDa.
Substrate: IKKtide (KKKKERLLDDRHDSG-LDSMKDEE); derived from human IkBA (amino acids 21–41).
Other: Reaction Buffer, DTT.

IKKα NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Anest, V. et al. (2003) A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423, 659–63.
2.Hoshino, K. et al. (2006) I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440, 949–53.

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