ADP-Glo? Kinase Assay + ACK Kinase Enzyme System

• 产品编号：V4051      品牌：promega       原厂货号：V4051
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

其它组分：

描述：

Recombinant human ACK (110–476) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs (1). Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality (2). Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC.
 

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
 

Kinase Enzyme System contains:
 

Kinase: ACK, 10μg (Human, recombinant; amino acids 110–476). MW: ~66kDa.
Substrate: Poly (4:1 Glu, Tyr) Peptide.
Other: Reaction Buffer, DTT, MnCl2.
 

ACK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
 

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
 

References

1.Manser, E. et al. (1993) A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363, 364–7.
2.van der Horst, E.H. (2005) Metastatic properties and genomic amplification of the tyrosine kinase gene ACK1. Proc. Nat. Acad. Sci. USA 102, 15901–6.

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