ADP-Glo? Kinase Assay + TRKB Kinase Enzyme System

• 产品编号：V4049      品牌：promega       原厂货号：V4049
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

其它组分：

描述：

Recombinant human TRKB (455–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TRKB is the high-affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations (1). TRKB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway (2). Signaling through TRKB leads to cell differentiation. Mutations in the TRKB gene have been associated with obesity and mood disorders.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:
Kinase: TRKB, 10μg (Human, recombinant; amino acids 455–end). MW: ~67kDa.
Substrate: Poly (4:1 Glu, Tyr) peptide.
Other: Reaction Buffer, DTT, MnCl2.

TRKB NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Gregory, T. et al. (1997) Signal transduction mediated by the truncated trkB receptor isoforms, trkB.T1 and trkB.T2.J. Neurosci. 17, 2683–90.
2.Pearse, R.N. et al. (2005) A neurotrophin axis in myeloma: TrkB and BDNF promote tumor-cell survival. Blood 105, 4429–36.

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