描述：
Recombinant full-length human PKC epsilon (PKCε) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCε is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCε is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may control anxiety-like behavior (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: PKCε, 10μg (Human, recombinant full-length). MW: ~110kDa.
Substrate: PKCtide (ERMRPRKRQGSVRRRV); derived from protein kinase C epsilon (amino acids 149–164).
Other: Reaction Buffer, DTT, Lipid Solution.
PKCε NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Features - Benefits
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Chen, C.H. et al. (1999) Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: role of epsilon protein kinase C. Proc. Nat. Acad. Sci. USA 96, 12784–9.
2.Hodge, C.W. et al. (2002) Decreased anxiety-like behavior, reduced stress hormones, and neurosteroid supersensitivity in mice lacking protein kinase C-epsilon. J. Clin. Invest. 110, 1003–10.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
