Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli, including growth factors, cytokines and phorbol esters. p70S6Kb contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. PI3 kinase pathway and mTOR are involved in the activation of p70S6Kb, but other pathways can also activate this target protein.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains: Kinase: p70S6Kb, 10μg (Human, recombinant full-length). MW: ~85kDa. Substrate: RSK substrate (KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acids 230–239). Other: Kinase Assay Buffer I, DTT.
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time. Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed. Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Gout, I. et al. (1998) Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region. J. Biol. Chem. 273, 30061–4.
2.Saitoh, M. et al. (1998) Cloning and characterization of p70(S6K-beta) defines a novel family of p70 S6 kinases. Biochem. Biophys. Res. Commun. 253, 470–6.
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