FMS Kinase Enzyme System

• 产品编号：V4022      品牌：promega       原厂货号：V4022
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

描述：

Recombinant human FMS (539–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 receptor (CSF1R). FMS is homodimeric, contains a so-called kinase insert domain and is a member of the CSF1R/PDGF receptor family of tyrosine-protein kinases. FMS mediates most if not all of the biological effects of CSF1, which control the production, differentiation and cell function of the monocyte/macrophage lineage (1). Mutations in FMS have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: FMS, 10μg (Human, recombinant; amino acids 539–end). MW: ~76kDa.
Substrate: Poly (4:1 Glu, Tyr) peptide.
Other: Reaction Buffer, DTT. MnCl2.

FMS NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Sherr, C.J. (1990) Regulation of mononuclear phagocyte proliferation by colony-stimulating factor-1. Int J Cell Cloning 8, 46–60.
2.Follows, G.A. et al. (2005) c-FMS chromatin structure and expression in normal and leukaemic myelopoiesis. Oncogene 24, 3643–51.

原厂资料：