ADP-Glo? Kinase Assay + CHK2 Kinase Enzyme System

产品编号：V4021 品牌：promega 原厂货号：V4021
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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1 each

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其它组分：

CHK2 Kinase Enzyme System【子货号：V4020，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type CHK2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative CHK2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: CHK2, 10μg (Human, recombinant full-length). MW: ~88kDa.
Substrate: Chktide (KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acids 205–225).
Other: Reaction Buffer, DTT.

CHK2 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Matsuoka, S. et al. (1998) Linkage of ATM to cell cycle regulation by the CHK2 protein kinase. Science 282, 1893–7.
2.Chehab, N.H. et al. (2000) Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 14, 278–88.