描述：
说明
在 Sf9 昆虫细胞中，我们通过杆状病毒 表达 N 端带 GST 标签的 重组 人源 ASK1 （第 649 位 - 第 946 位氨基酸）。 ASK1 也被称为 MAPKKK5 ，可以激活 MKK3 、 MKK4 (SEK1) 、以及 MKK6 。 ASK1 的过表达会诱导 凋亡 性细胞死亡 ；在肿瘤坏死因子 α 处理的细胞中， ASK1 能被激活。 ASK1 与 TRAF 家族成员相互作用，并被 TNF 信号通路中的 TRAF2 激活；被 TRAF2 激活后， ASK1 激活 MKK4 ， MKK4 再激活 JNK ；因此， ASK1 是 TRAF2 诱导的 JNK 激活途径的媒介。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
• 激酶： ASK1 ， 10μg ( 人源，重组， 第 649 位 - 第 946 位氨基酸 ) ； MW : ~ 60 kDa 。
• 底物： 天然的猪髓磷脂碱性 蛋白（ MBP ） 。
其它组分： 反应缓冲液， DTT 。
ASK1 NCBI 数据库入口。
人类激酶酶系统及其应用。
特点
• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
• 完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2 。
• 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册
ASK1 Kinase Enzyme System Product Information
储存条件
收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。
参考文献 1. Ichijo, H. et al. (1997) Science 275 , 90–4.
2. Nishitoh, H. et al. (1998) Molec. Cell 2 , 389–95.
专利/免责声明
For Cat.# V9481:
Patents Pending.
U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending.
U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending.
U.S. Pat. No. 7,741,067 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
原厂资料：
Recombinant human ASK1 (649–946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha. ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: ASK1, 10μg (Human, recombinant; amino acids 649–946). MW: ~60kDa.
Substrate: Native Swine Myelin Basic Protein (MBP).
Other: Reaction Buffer, DTT.
ASK1 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Features - Benefits
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Ichijo, H. et al. (1997) Science 275, 90–4.
2.Nishitoh, H. et al. (1998) Molec. Cell 2, 389–95.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
