Yaghmaie, F. et al. (2007) Experimental Gerontology 42(1–2) , 148–9.
Jones, H. et al. (2004) Endocr. Relat. Cancer 11(4) , 793–814.
专利/免责声明
For Cat.# V9401:
Patents Pending.
U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending.
U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
原厂资料：
Recombinant human IGF1R (amino acids 960–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. IGF1R (Insulin-like Growth Factor 1) is a transmembrane tyrosine kinase receptor that is activated by IGF-1 and by the related growth factor IGF-2. IGF1R mediates the effects of IGF-1 and plays an important role in growth and anabolic effects in adults. The IGF1R is implicated in several cancers, most notably breast cancer, where it is highly overexpressed and functions as an anti-apoptotic agent by enhancing cell survival. In many instances, the anti-apoptotic properties of IGF1R overexpression allow cancerous cells to resist the cytotoxic properties of chemotherapeutic drugs or radiotherapy.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: IGF1R, 10μg (Human, recombinant; amino acids 960–end). MW: ~53kDa. Substrate: IGF1Rtide (KKKSPGEYVNIEFG); derived from human IRS-1 protein residues 891–902. Other: Reaction Buffer, DTT, MnCl2.
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time. Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed. Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Yaghmaie, F. et al. (2007) Experimental Gerontology 42(1–2), 148–9.
2.Jones, H. et al. (2004) Endocr. Relat. Cancer 11(4), 793–814.
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