1. Leibersperger, H. et al. (1991) J. Biol Chem. 266(22) , 14778–84.
2. Mischak, H. et al. (1991) Biochemistry 30(32) , 7925–31.
专利/免责声明
For Cat.# V9721:
Patents Pending.
U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending.
U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
原厂资料：
Recombinant full-length human PKC delta (PKCδ) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79kDa protein kinase that shows strict dependence on the presence of phospholipids but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKCδ. Northern blot analysis indicated that PKCδ is widely distributed in almost all tissues and is a major isoform of PKC expressed in hemopoietic cells. PKCδ is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains: Kinase: PKCδ, 10μg (Human, recombinant full-length). MW: ~104kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109–121). Other: Reaction Buffer, DTT, Lipid solution.
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time. Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed. Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Leibersperger, H. et al. (1991) J. Biol Chem. 266(22), 14778–84.
2.Mischak, H. et al. (1991) Biochemistry 30(32), 7925–31.
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