1. Collett, M.S. et al. (1978) Proc. Natl. Acad. Sci. USA 75(4) , 2021–4.
2. Jacobs, C. et al. (1983) Cancer Res. 43(4) , 1696–702.
专利/免责声明
For Cat.# V9741:
Patents Pending.
U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending.
U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
原厂资料：
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC family belongs to nonreceptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. SRC is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains: Kinase: SRC, 10μg (Human, recombinant full-length). MW: ~83kDa. Substrate: SRC substrate (KVEKIGEGTYGVVYK-amide); derived from human p34cdc2 (amino acids 6–20). Other: Reaction Buffer, DTT, MnCl2.
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time. Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed. Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Collett, M.S. et al. (1978) Proc. Natl. Acad. Sci. USA 75(4), 2021–4.
2.Jacobs, C. et al. (1983) Cancer Res. 43(4), 1696–702.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
