1.Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29(6) , 385–413.
2.Edelmann, H.M. et al. (1996) J. Biol. Chem. 271(2) , 963–71.
专利/免责声明
For Cat.# V9611:
Patents Pending.
U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending.
U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
原厂资料：
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation, and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine-389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Kinase: p70S6K, 10μg (Human, recombinant full-length). MW: ~76kDa. Substrate: S6K substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acid 230–238). Other: Reaction Buffer, DTT.
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time. Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed. Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
Notes
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
References
1.Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29(6), 385–413.
2.Edelmann, H.M. et al. (1996) J. Biol. Chem. 271(2), 963–71.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
